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iodixanol step gradient column optiprep  (Millipore)


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    Millipore iodixanol step gradient column optiprep
    Iodixanol Step Gradient Column Optiprep, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/optiprep+step+gradient/iodixanol+step+gradient+column+optiprep/pm37272896-37-7-12
    Average 90 stars, based on 1 article reviews
    iodixanol step gradient column optiprep - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Fractionation:

    Article Title: Lysosomal Proteolysis and Autophagy Require Presenilin 1 and Are Disrupted by Alzheimer-Related PS1 Mutations
    Article Snippet: .. For subcellular fractionation, homogenate (0.5 ml) was layered on top of an Optiprep (Sigma) step gradient (10, 15, 20, 25, and 30%, 2.3 ml each) into polyallomer tubes (Beckman) and centrifuged in a SW-40Ti rotor with a model L8–80M Beckman ultracentrifuge (100,000 g, 16 hours, 4°C). ..

    Purification:

    Article Title: Antibody Competition Reveals Surface Location of HPV L2 Minor Capsid Protein Residues 17–36
    Article Snippet: Particles were matured by incubating cell lysates at 37 °C for 24 h and subsequently treating with Benzonase (Sigma-Aldrich, St. Louis, MO, USA) and Plasmid Safe (Epicentre, Madison, WI, USA). .. Purification of the resulting particles was performed by ultracentrifugation of the lysates at 234,000× g for 3.5 h on an Optiprep step gradient (Sigma-Aldrich, St. Louis, MO, USA). ..



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    Isolation of microglia from adult rat mesencephalon using <t>density</t> <t>gradient</t> fractionation. (A) Cellular pool from the midbrain of 10-week-old Wistar rat using <t>OptiPrep</t> step gradient separation method. The fraction and cellular designation were based on the previous study . Note the pellet at the bottom that contains microglia. (B) Double immunocytofluorescence labeling of Hoechst (nucleus) and one of the microglial (OX42), astrocytic (GFAP), and neuronal (NeuN) markers using primary cultured cells obtained from the pellet in (A) . Scale bar represents 100 μm. (C) Semi-quantification of the number of OX42-ir, GFAP-ir, and NeuN-ir cells against Hoechst positive nuclei. The results are expressed as the percentage of the number of OX42-ir, GFAP-ir, and NeuN-ir cells to the total number of Hoechst-ir nuclei. * P < 0.05 compared with value from OX42-ir result (ANOVA with post hoc Student’s t test). (D) Immunocytofluorescence of OX6 and iNOS in primary cultured microglia originated from the contralateral (Cont) or ipsilateral (Ipsi) SN at 7 dpl. Microglia cultivated from the ipsilateral SN are highly immunoreactive with OX6 and iNOS, meaning that they are highly activated and produce high level of reactive nitrogen species. Scale bar represents 20 μm.
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    optiprep step gradient  (Millipore)
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    Isolation of microglia from adult rat mesencephalon using <t>density</t> <t>gradient</t> fractionation. (A) Cellular pool from the midbrain of 10-week-old Wistar rat using <t>OptiPrep</t> step gradient separation method. The fraction and cellular designation were based on the previous study . Note the pellet at the bottom that contains microglia. (B) Double immunocytofluorescence labeling of Hoechst (nucleus) and one of the microglial (OX42), astrocytic (GFAP), and neuronal (NeuN) markers using primary cultured cells obtained from the pellet in (A) . Scale bar represents 100 μm. (C) Semi-quantification of the number of OX42-ir, GFAP-ir, and NeuN-ir cells against Hoechst positive nuclei. The results are expressed as the percentage of the number of OX42-ir, GFAP-ir, and NeuN-ir cells to the total number of Hoechst-ir nuclei. * P < 0.05 compared with value from OX42-ir result (ANOVA with post hoc Student’s t test). (D) Immunocytofluorescence of OX6 and iNOS in primary cultured microglia originated from the contralateral (Cont) or ipsilateral (Ipsi) SN at 7 dpl. Microglia cultivated from the ipsilateral SN are highly immunoreactive with OX6 and iNOS, meaning that they are highly activated and produce high level of reactive nitrogen species. Scale bar represents 20 μm.
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    Image Search Results


    Isolation of microglia from adult rat mesencephalon using density gradient fractionation. (A) Cellular pool from the midbrain of 10-week-old Wistar rat using OptiPrep step gradient separation method. The fraction and cellular designation were based on the previous study . Note the pellet at the bottom that contains microglia. (B) Double immunocytofluorescence labeling of Hoechst (nucleus) and one of the microglial (OX42), astrocytic (GFAP), and neuronal (NeuN) markers using primary cultured cells obtained from the pellet in (A) . Scale bar represents 100 μm. (C) Semi-quantification of the number of OX42-ir, GFAP-ir, and NeuN-ir cells against Hoechst positive nuclei. The results are expressed as the percentage of the number of OX42-ir, GFAP-ir, and NeuN-ir cells to the total number of Hoechst-ir nuclei. * P < 0.05 compared with value from OX42-ir result (ANOVA with post hoc Student’s t test). (D) Immunocytofluorescence of OX6 and iNOS in primary cultured microglia originated from the contralateral (Cont) or ipsilateral (Ipsi) SN at 7 dpl. Microglia cultivated from the ipsilateral SN are highly immunoreactive with OX6 and iNOS, meaning that they are highly activated and produce high level of reactive nitrogen species. Scale bar represents 20 μm.

    Journal: BMC Neuroscience

    Article Title: Down-regulation of microglial activity attenuates axotomized nigral dopaminergic neuronal cell loss

    doi: 10.1186/1471-2202-14-112

    Figure Lengend Snippet: Isolation of microglia from adult rat mesencephalon using density gradient fractionation. (A) Cellular pool from the midbrain of 10-week-old Wistar rat using OptiPrep step gradient separation method. The fraction and cellular designation were based on the previous study . Note the pellet at the bottom that contains microglia. (B) Double immunocytofluorescence labeling of Hoechst (nucleus) and one of the microglial (OX42), astrocytic (GFAP), and neuronal (NeuN) markers using primary cultured cells obtained from the pellet in (A) . Scale bar represents 100 μm. (C) Semi-quantification of the number of OX42-ir, GFAP-ir, and NeuN-ir cells against Hoechst positive nuclei. The results are expressed as the percentage of the number of OX42-ir, GFAP-ir, and NeuN-ir cells to the total number of Hoechst-ir nuclei. * P < 0.05 compared with value from OX42-ir result (ANOVA with post hoc Student’s t test). (D) Immunocytofluorescence of OX6 and iNOS in primary cultured microglia originated from the contralateral (Cont) or ipsilateral (Ipsi) SN at 7 dpl. Microglia cultivated from the ipsilateral SN are highly immunoreactive with OX6 and iNOS, meaning that they are highly activated and produce high level of reactive nitrogen species. Scale bar represents 20 μm.

    Article Snippet: The collected supernatant was loaded upon the top of the prepared OptiPrep step gradient (Sigma-Aldrich Co., St. Louis, USA) solution.

    Techniques: Isolation, Fractionation, Labeling, Cell Culture