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iodixanol step gradient column optiprep  (Millipore)


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    Millipore iodixanol step gradient column optiprep
    Iodixanol Step Gradient Column Optiprep, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/iodixanol step gradient column optiprep/product/Millipore
    Average 90 stars, based on 1 article reviews
    iodixanol step gradient column optiprep - by Bioz Stars, 2026-05
    90/100 stars

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    5-step optiprep gradient consisted of 40, 30, 20, 10, and 5% iodixanol  (Millipore)
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    Isolation of microglia from adult rat mesencephalon using <t>density</t> <t>gradient</t> fractionation. (A) Cellular pool from the midbrain of 10-week-old Wistar rat using <t>OptiPrep</t> step gradient separation method. The fraction and cellular designation were based on the previous study . Note the pellet at the bottom that contains microglia. (B) Double immunocytofluorescence labeling of Hoechst (nucleus) and one of the microglial (OX42), astrocytic (GFAP), and neuronal (NeuN) markers using primary cultured cells obtained from the pellet in (A) . Scale bar represents 100 μm. (C) Semi-quantification of the number of OX42-ir, GFAP-ir, and NeuN-ir cells against Hoechst positive nuclei. The results are expressed as the percentage of the number of OX42-ir, GFAP-ir, and NeuN-ir cells to the total number of Hoechst-ir nuclei. * P < 0.05 compared with value from OX42-ir result (ANOVA with post hoc Student’s t test). (D) Immunocytofluorescence of OX6 and iNOS in primary cultured microglia originated from the contralateral (Cont) or ipsilateral (Ipsi) SN at 7 dpl. Microglia cultivated from the ipsilateral SN are highly immunoreactive with OX6 and iNOS, meaning that they are highly activated and produce high level of reactive nitrogen species. Scale bar represents 20 μm.
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    optiprep step gradient  (Millipore)
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    Isolation of microglia from adult rat mesencephalon using <t>density</t> <t>gradient</t> fractionation. (A) Cellular pool from the midbrain of 10-week-old Wistar rat using <t>OptiPrep</t> step gradient separation method. The fraction and cellular designation were based on the previous study . Note the pellet at the bottom that contains microglia. (B) Double immunocytofluorescence labeling of Hoechst (nucleus) and one of the microglial (OX42), astrocytic (GFAP), and neuronal (NeuN) markers using primary cultured cells obtained from the pellet in (A) . Scale bar represents 100 μm. (C) Semi-quantification of the number of OX42-ir, GFAP-ir, and NeuN-ir cells against Hoechst positive nuclei. The results are expressed as the percentage of the number of OX42-ir, GFAP-ir, and NeuN-ir cells to the total number of Hoechst-ir nuclei. * P < 0.05 compared with value from OX42-ir result (ANOVA with post hoc Student’s t test). (D) Immunocytofluorescence of OX6 and iNOS in primary cultured microglia originated from the contralateral (Cont) or ipsilateral (Ipsi) SN at 7 dpl. Microglia cultivated from the ipsilateral SN are highly immunoreactive with OX6 and iNOS, meaning that they are highly activated and produce high level of reactive nitrogen species. Scale bar represents 20 μm.
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    Image Search Results


    Isolation of microglia from adult rat mesencephalon using density gradient fractionation. (A) Cellular pool from the midbrain of 10-week-old Wistar rat using OptiPrep step gradient separation method. The fraction and cellular designation were based on the previous study . Note the pellet at the bottom that contains microglia. (B) Double immunocytofluorescence labeling of Hoechst (nucleus) and one of the microglial (OX42), astrocytic (GFAP), and neuronal (NeuN) markers using primary cultured cells obtained from the pellet in (A) . Scale bar represents 100 μm. (C) Semi-quantification of the number of OX42-ir, GFAP-ir, and NeuN-ir cells against Hoechst positive nuclei. The results are expressed as the percentage of the number of OX42-ir, GFAP-ir, and NeuN-ir cells to the total number of Hoechst-ir nuclei. * P < 0.05 compared with value from OX42-ir result (ANOVA with post hoc Student’s t test). (D) Immunocytofluorescence of OX6 and iNOS in primary cultured microglia originated from the contralateral (Cont) or ipsilateral (Ipsi) SN at 7 dpl. Microglia cultivated from the ipsilateral SN are highly immunoreactive with OX6 and iNOS, meaning that they are highly activated and produce high level of reactive nitrogen species. Scale bar represents 20 μm.

    Journal: BMC Neuroscience

    Article Title: Down-regulation of microglial activity attenuates axotomized nigral dopaminergic neuronal cell loss

    doi: 10.1186/1471-2202-14-112

    Figure Lengend Snippet: Isolation of microglia from adult rat mesencephalon using density gradient fractionation. (A) Cellular pool from the midbrain of 10-week-old Wistar rat using OptiPrep step gradient separation method. The fraction and cellular designation were based on the previous study . Note the pellet at the bottom that contains microglia. (B) Double immunocytofluorescence labeling of Hoechst (nucleus) and one of the microglial (OX42), astrocytic (GFAP), and neuronal (NeuN) markers using primary cultured cells obtained from the pellet in (A) . Scale bar represents 100 μm. (C) Semi-quantification of the number of OX42-ir, GFAP-ir, and NeuN-ir cells against Hoechst positive nuclei. The results are expressed as the percentage of the number of OX42-ir, GFAP-ir, and NeuN-ir cells to the total number of Hoechst-ir nuclei. * P < 0.05 compared with value from OX42-ir result (ANOVA with post hoc Student’s t test). (D) Immunocytofluorescence of OX6 and iNOS in primary cultured microglia originated from the contralateral (Cont) or ipsilateral (Ipsi) SN at 7 dpl. Microglia cultivated from the ipsilateral SN are highly immunoreactive with OX6 and iNOS, meaning that they are highly activated and produce high level of reactive nitrogen species. Scale bar represents 20 μm.

    Article Snippet: The collected supernatant was loaded upon the top of the prepared OptiPrep step gradient (Sigma-Aldrich Co., St. Louis, USA) solution.

    Techniques: Isolation, Fractionation, Labeling, Cell Culture